A modified method for protein separation by zone electrophoresis on a starch gel.
نویسندگان
چکیده
In the present paper (1) a method of preparative zone electrophoresis (cf. 2, 3) is described in which a stiff starch gel with only 7 to 8 per cent of solid constituents is used as a supporting medium. Two simple devices will be described, one for the fractionation of 2 ml. of protein solution and the other for processing up to 50 ml. of solution, containing 5 to 10 per cent of protein mixture. Both devices have been used successfully in our laboratory, and some of the results obtained with them, in particular the fractionation of plasma proteins from tumor-bearing mice, will be presented in this communication.
منابع مشابه
A Simpler and Faster Version of Two-Dimensional Gel Electrophoresis Using Vertical, Mini SDS-PAGE Apparatus
We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...
متن کاملComparison of the serum Protein fractions of the developing chickens embryo by the technique of Starch-gel Electrophoresis
متن کامل
Starch-gel electrophoresis of rat-serum proteins.
In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...
متن کاملDisc Electrophoresis. Ii. Method and Application to Human Serum Proteins.
The purpose of this paper is to describe a technique of zone electrophoresis in polyacrylamide gels. The salient characteristics of the technique reside in (1) the controlled variation of the gel pore size for the purpose of increasing the resolution of ions based on dimensional differences and (2) an electrophoretic step for concentrating the sample ions into a narrow starting zone prior to el...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 220 2 شماره
صفحات -
تاریخ انتشار 1956