A modified method for protein separation by zone electrophoresis on a starch gel.

نویسندگان

  • P BERNFELD
  • J S NISSELBAUM
چکیده

In the present paper (1) a method of preparative zone electrophoresis (cf. 2, 3) is described in which a stiff starch gel with only 7 to 8 per cent of solid constituents is used as a supporting medium. Two simple devices will be described, one for the fractionation of 2 ml. of protein solution and the other for processing up to 50 ml. of solution, containing 5 to 10 per cent of protein mixture. Both devices have been used successfully in our laboratory, and some of the results obtained with them, in particular the fractionation of plasma proteins from tumor-bearing mice, will be presented in this communication.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 220 2  شماره 

صفحات  -

تاریخ انتشار 1956